Fig. 2

ATF2 promotes cell proliferation and invasion in LUAD cells. a The knockdown efficiency of three shRNA was detected by qRT-PCR in A549 and H1299 cells. b The knockdown efficiency of the shRNA for following experiments was validated by western blotting. c Colony formation assay was conducted following ATF2 knockdown in LUAD cells. d, e Cell viability was detected by CCK8 assay following ATF2 knockdown in A549 and H1299 cells. f Invasion ability of LUAD cells was determined by transwell invasion assay following ATF2 knockdown. g The expression of ATF2 protein was detected by western blotting after transfection with ATF2-overexpressing vector and empty vector in LUAD cells. h Colony formation assay was conducted following ATF2 overexpression in LUAD cells. i, j Cell viability was detected by CCK8 assay following ATF2 overexpression in A549 and H1299 cells. k Invasion ability of LUAD cells was detected by transwell invasion assay following ATF2 overexpression in LUAD cells. All data are shown as the mean ± SD of three independent experiments. ^*P < 0.05, ^**P < 0.01