Fig. 3

ATF2 activates NEAT1 transcription in LUAD cells. a NEAT1 expression in LUAD tissues and normal lung tissues from TCGA database. Normal, n = 59, LUAD, n = 533. b NEAT1 expression in primary and recurrent LUAD tissues from TCGA database. Primary, n = 513, Recurrence, n = 20. c NEAT1 expression in LUAD and normal lung tissues was detected by qRT-PCR. Normal adjacent lung tissues (NAT), n = 25, LUAD, n = 31. d NEAT1 expression in LUAD and normal bronchial epithelial cell lines was detected by qRT-PCR. e A positive correlation between NEAT1 and ATF2 expression in LUAD tissues was identified by qRT-PCR analysis. (0.2 ≤|r|< 0.4, low linear correlation). f Schematic showing the putative ATF2-binding site in the NEAT1 promoter region. g Luciferase activity assay was performed in A549 cells after transfection as indicated. h ChIP assay was performed for detecting ATF2 occupancy on the NEAT1 promoter region. i, j Relative expression of NEAT1 was detected by qRT-PCR in LUAD cells after transfection as indicated. ATF2-mut-in, the shRNA-insensitive mutant ATF2 plasmid. All data are shown as the mean ± SD of three independent experiments. ^*P < 0.05, ^**P < 0.01