Fig. 6

NEAT1 is a direct target of miR-26a in LUAD cells. a Four online tools (miRanda, Targetscan, Pictar and StarBase) were used to predict the miRNAs that target both NEAT1 and ATF2 3′UTR. b The expression of the five predicted miRNAs in LUAD tissues and normal lung tissues was analyzed using GSE48414 dataset. Normal, n = 20, LUAD, n = 154. c MiR-26a-5p, miR-26b-5p and miR-204-5p levels in A549 cells were detected by qRT-PCR following NEAT1 knockdown. d MiR-26a-5p expression in H1299 cells was detected by qRT-PCR following NEAT1 knockdown. e miR-26a-5p expression in LUAD cells was detected by qRT-PCR following NEAT1 overexpression. f, g NEAT1 expression was detected by qRT-PCR in LUAD cells after transfection with miR-26a-5p mimics and inhibitors, respectively. h Schematic representation of the putative binding site between miR-26a-5p and NEAT1. i Luciferase activity assay was performed in A549 cells after transfection as indicated. All data are shown as the mean ± SD of three independent experiments. ^*P < 0.05, ^**P < 0.01