Fig. 7

NEAT1 positively regulates ATF2 expression via sponging miR-26a-5p in LUAD cells. a Schematic representation of the putative binding site between miR-26a-5p and ATF2. b Luciferase activity assay was performed in A549 cells after transfection as indicated. c, d Relative expression levels of ATF2 mRNA in LUAD cells were detected by qRT-PCR after transfection with miR-26a-5p mimics and inhibitors, respectively. e ATF2 protein levels were detected by western blotting after transfection as indicated. f Luciferase activity assay was performed in A549 cells after transfection as indicated. g RNA immunoprecipitation assay was performed to determine the amount of NEAT1, ATF2 and miR-26a-5p pulled down by anti-Ago2 antibody in A549 and H1299 cells. All data are shown as the mean ± SD of three independent experiments. ^*P < 0.05, ^**P < 0.01