Fig. 10
From: LncRNA PCAT6 regulates the progression of pituitary adenomas by regulating the miR-139-3p/BRD4 axis
SiBRD4 reversed the promoting effect of miR-139-3p inhibitor on BRD4 expression, and the viability and migration of PA cells. a, c The expressions of BRD4 in RC-4B/C and GH3 cells after transfection were detected by RT-qPCR. GAPDH was used as an internal control. b, d The expressions of BRD4 in RC-4B/C and GH3 cells after transfection were detected by Western blot. GAPDH was used as an internal control. e, f The viability of RC-4B/C and GH3 cells after transfection was detected by CCK-8 assay. g–i The migration of RC-4B/C and GH3 cells after transfection was detected by wound healing assay (Magnification × 100). (**P < 0.01, ***P < 0.001, vs. IC + siNC; ###P < 0.001, vs. I + siNC; ^P < 0.05, ^^^P < 0.001, vs. I + siBRD4). I miR-139-3p inhibitor, IC inhibitor control, siNC small interfering RNA negative control