Fig. 9
From: LncRNA PCAT6 regulates the progression of pituitary adenomas by regulating the miR-139-3p/BRD4 axis
BRD4 was high-expressed in IPA and was targeted by miR-139-3p in PA cells. a The mRNAs that were possibly targeted by miR-139-3p were then predicted by miRDB, TargetScan, and TarBase v.8. b The putative binding site between miR-139-3p and SDCBP or BRD4 was predicted by Targetscan7.2. c, d The results of luciferase assay validated that miR-139-3p targeted SDCBP in PA cells RC-4B/C and GH3. e, f The results of luciferase assay validated that miR-139-3p targeted BRD4 in PA cells RC-4B/C and GH3. g The expressions of BRD4 in normal pituitary and IPA tissues were detected by RT-qPCR. GAPDH was used as an internal control. h, j The correlation between BRD4 expression and miR-139-3p expression in normal pituitary and IPA tissues was analyzed by Pearson correlation analysis. i, k The correlation between BRD4 expression and PCAT6 expression in normal pituitary and IPA tissues was analyzed by Pearson correlation analysis. (^P < 0.05, ^^^P < 0.001, vs. MC). IPA invasive pituitary adenomas, MC mimic control