Fig. 6

The targeted relationships among circPIP5K1A, TCF12 and miR-515-5p. a The miRNA target of circPIP5K1A and TCF12 was analyzed through Starbase, and a total of 22 miRNAs were common targets of CircPIP5K1A and TCF12. b RT-PCR was used to determine the levels of the 22 miRNAs in circPIP5K1A overexpressed cell. c The miR-515-5p expression in glioma tissues and adjacent normal tissues was detected by RT-PCR. d K-M assay was used to analyze the relationship between miR-515-5p expression with glioma prognosis. e, f Person correlation analysis was adopted to detect the correlation between miR-515-5p and circPIP5K1A, miR-515-5p and TCF12 in the tissues of glioma patients. g Relative circPIP5K1A level in U87 cells lysates captured by biotin-labeled miR-515-5p or NC was detected by RNA pull-down assay. H: The binding relationships of miR-515-5p with both circPIP5K1A and TCF12 was shown. i, j RIP assay was employed to examine the bindings of CircPIP5K1A and TCF12 with ant-Ago2 antibody in U87 cells. k, l Dual-luciferase reporter assay was analyzed to clarify the targeting binding relationships between miR-515-5p and circPIP5K1A, miR-515-5p and TCF12 in U87 cells. Ns P > 0.05, **P < 0.01, ***P < 0.001