Fig. 4

RT-qPCR and Western blot analysis results show decreased miR-29b expression and increased ETV4 expression in CRC tissues. A, miR-29b expression (normalized to U6) and mRNA expression of E-cadherin, TSP-1, ETV4, ERK1/2, MMP-2, MMP-9, Vimentin, and VEGF (normalized to GAPDH) in CRC and adjacent normal tissues determined using RT-qPCR. B, Western blot analysis of E-cadherin, TSP-1, ETV4, ERK1/2, MMP-2, MMP-9, Vimentin, and VEGF proteins (normalized to GAPDH) in CRC and adjacent normal tissues. *p < 0.05 compared with adjacent normal tissues. Each experiment was repeated 3 times independently. Data between the two groups were compared by paired t-test