Fig. 5

LINC00342 functions as a ceRNA of NPEPL1 by sponging miR-19a-3p. TargetScan was used to predict that NPEPL1 mRNA 3′UTR contains miR-19a-3p binding site, and the mutational 3′UTR of NPEPL1 mRNA were indicated (a); SW480 and HT-29 cells were co-transfected with the wild or mutant type NPEPL1 plasmids and miR-19a-3p mimic or mimic NC, and the luciferase activities were measured (b); ^**P < 0.01 vs. mimic NC group. RIP assay showed that NPEPL1 was a direct target of miR-19a-3p (c); The mRNA and protein levels of NPEPL1 in SW480 and HT-29 cells transfected with mimic NC or miR-19a-3p mimic (d, e); **P < 0.01 vs. mimic NC group. Luciferase reporter assay showed the interaction between LINC00342, miR-19a-3p and NPEPL1 (f); **P < 0.01 vs. NC mimic group. ^##P < 0.05 vs. miR-19a-3p mimic group. The mRNA and protein levels of NPEPL1 in SW480 and HT-29 cells transfected with sh-NC or sh-LINC00342 (g, h); **P < 0.01 vs. sh-NC group