Fig. 4

Silenced SNHG14 increases miR-211-3p expression and decreases ESM1 expression; elevated miR-211-3p decreases ESM1 expression in BCa cells. a The efficiency of SNHG14 and miR-211-3p in T24 cells was verified by RT-qPCR; b ESM1 mRNA expression in T24 cells detected via RT-qPCR; c Protein bands of ESM1 in T24 cells tested via western blot analysis; d The expression of ESM1 protein in T24 cells detected via western blot analysis; e The efficiency of SNHG14 and miR-211-3p in UMUC-3 cells was verified by RT-qPCR; f ESM1 mRNA expression in UMUC-3 cells detected via RT-qPCR; g Protein bands of ESM1 in UMUC-3 cells tested via western blot analysis; h The expression of ESM1 protein in UMUC-3 cells detected via western blot analysis. * vs the si-NC group, P < 0.05; ^ vs the mimic NC group, P < 0.05; and vs the si-SNHG14 + inhibitor NC group, P < 0.05; a vs the oe-NC group, P < 0.05; b vs the inhibitor NC group, P < 0.05; c vs the oe-SNHG14 + mimic NC group, P < 0.05. The experiment was repeated independently for 3 times, and the measurement data were expressed as mean ± standard deviation. One-way ANOVA was functioned for comparison among multiple groups, and Tukey’s post hoc test was used for pairwise comparison