Fig. 2

ADAM10 is down-regulated in MDA-MB-231 cells with the use of ADAM10 siRNA. a Knockdown efficiency was determined by qRT-PCR after 24, 48 and 72 h transfecting ADAM10 siRNA in MDA-MB-231 cells. GAPDH was used as a loading control. Bars represent the mean of triplicate samples; error bars represent SD. Data are representative of three independent experiments. The significant difference between MDA-MB-231 and MDA-MB-231 with siControl or siADAM10 is indicated by *p < 0.05, **p < 0.01. b Knockdown efficiency was determined by western blot after 48 and 96 h transfecting ADAM10 siRNA in MDA-MB-231 cells. GAPDH was used as a loading control. Quantitative analysis of band intensities was conducted in western blot analyses. Data are shown as mean ± SEM of three independent experiments