Fig. 1

Circ-CCDC66 is upregulated in cervical cancer tissues. a The expression of circ-CCDC66 in 36 pairs of cervical cancer tissues and its matched normal tissues were analyzed by qRT-PCR. b and c relative expression of CCDC66 was analyzed according to TNM stage and tumor size using qRT-PCR. d relative expression of circ-CCDC66 in cervical cancer cell lines HeLa, SiHa, C33A, HT-3, and normal cervical cell line H8 were assessed by qRT-PCR. e nuclear-cytoplasmic fractionation assays were conducted to detect the expression of CCDC66 in the nuclear or cytoplasmic of cervical cells. f The location of CCDC66 in cervical cells were detected by FISH assay. Data were presented as mean ± SD, all experiments were repeated at least three times. Scare bar for FISH image: 20 μm. *P < 0.05, **P < 0.01