Table 1 Primer sequences
Gene | Forward (5ʹ-3ʹ) | Reverse (5ʹ-3ʹ) |
|---|---|---|
Homo-TCEAL7 | GAGCTGACGTGAACCGAAGT | GGAGGAAGGAGCTATCTGTGG |
AKT1 | GAAGACGGGAGCAGGCG | TGTACTCCCCTCGTTTGTGC |
AKT2 | CTGCCACCATGAATGAGGTGAA | CATGGTCACTTTAGCCCGTG |
STAT3 | CTGTGGGAAGAATCACGCCT | ACATCCTGAAGGTGCTGCTC |
β-catenin | CTGAGGAGCAGCTTCAGTCC | GGCCATGTCCAACTCCATCA |
TGF-β | GGGCTACCATGCCAACTTCT | GACACAGAGATCCGCAGTCC |
YAP | CCCTCGTTTTGCCATGAACC | GTTGCTGCTGGTTGGAGTTG |
c-Myc | GCAATGCGTTGCTGGGTTAT | CGCATCCTTGTCCTGTGAGT |
FOXO1 | TTGAACCCACACGGTGGTAG | CCTCGGCCTCTGAAACACTT |
GAPDH | TGCACCACCAACTGCTTAGC | GGCATGGACTGTGGTCATGAG |
Mus-TCEAL7 | AGGGCTCTGCATTCTAACCG | TCACTGCTTTCCTAACGCGA |
AKT1 | CCGCCTGATCAAGTTCTCCT | CAGACCCATGAGCCCACATT |
AKT2 | GTGGCTGGAAAAGGCGGTAT | TTCACACGCTGTCACCTAGC |
c-Myc | GTTGGAAACCCCGCAGACAG | GTAGCGACCGCAACATAGGA |
GAPDH | AGAGAGGCCCAGCTACTCG | CAAATCCGTTCACACCGACC |
miR-758 | ACACTCCAGCTGGGAACGATG | CTCAACTGGTGTCGTGGAGTCGGCA |
U6 | CTCGCTTCGGCAGCACA | AACGCT TCACGAATTTGCGT |