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Fig. 1 | Cancer Cell International

Fig. 1

From: CTCF-silenced miR-137 contributes to EMT and radioresistance in esophageal squamous cell carcinoma

Fig. 1

MiR-137 is expressed at a low level in ESCC cells and suppresses cell proliferation, migration and radioresistance. a The expression of miR-137 was evaluated by qRT-PCR assay in ESCC cell lines (Eca109, EC9706, KYSE150, KYSE450) and normal esophageal epithelial cell line (Het-1A). b The overexpression efficiency of miR-137 mimics was assessed by qRT-PCR. c The proliferation ability was testified by EdU assay in Eca109 and KYSE450 cells transfected with miR-137 mimics or NC mimics. d, e Transwell assays were performed to evaluate the migratory and invasive capacities after transfected with miR-137 mimics. f Western blot assays were conducted to measure the level of EMT-related proteins in two ESCC cells with miR-137 upregulation. g The expression of miR-137 was detected by qRT-PCR assay in parental Eca109 and KYSE450 cells and radio-resistant cells. h Clonogenic survival assay showed that the surviving fraction of radio-resistant cells and parental cells. i Flow cytometry analysis was performed to evaluate cell apoptosis rate in parental and radio-resistant cells. j Clonogenic survival assay was performed to testify radio sensitivity after transfected with miR-137 mimics. k Flow cytometry analysis was performed to evaluate cell apoptosis rate after transfected with miR-137 mimics. **P < 0.01

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