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Fig. 3 | Cancer Cell International

Fig. 3

From: CTCF-silenced miR-137 contributes to EMT and radioresistance in esophageal squamous cell carcinoma

Fig. 3

EZH2 and PXN are two downstream targets of miR-137. a The putative targets of miR-137 were predicted by utilizing starBase and miRTarBase bioinformatics tools. b Results of qRT-PCR analysis showed the mRNAs that were downregulate by the miR-137 upregulation in Eca109 and KYSE450 cells. c qRT-PCR was performed to detect the expressions of target genes in four ESCC cell lines and the normal cell line. d qRT-PCR was performed to evaluate the expressions of EZH2, PXN and RNF4 in parental and radio-resistant cells. e RIP assay manifested that miR-137, EZH2 and PXN were significantly enriched in Ago2 group. f RNA pull down showed that Bio-miR-137-WT could pull down EZH2 and PXN. g The binding sites between miR-137 and EZH2 or PXN. h Luciferase reporter assay was performed to validate the interaction between miR-137 and EZH2 or PXN. i The expression of PXN or EZH2 was measured by qRT-PCR in cells transfected with NC mimics, miR-137 mimics or miR-137 mimics + CTCF. **P < 0.01

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