Fig. 1

SEC61A1 promotes HCC cell proliferation, migration and stemness. a SEC61A1 expression in HCC cell lines and normal liver epithelial cell line was evaluated through qRT-PCR. b SEC61A1 expression was decreased in HCC cells transfected with sh-SEC61A1#1/2. c, d Proliferation ability of SEC61A1-silenced HCC cells was evaluated through EdU assay and colony formation assay. e Transwell assay examined the migration ability of HCC cells transfected with sh-SEC61A1#1/2 or sh-NC. f Sphere formation assay detected the sphere formation efficiency in SEC61A1-silenced HCC cells. g Stemness markers, including OCT4, Nanog and SOX2 were detected at the RNA level through qRT-PCR. *P < 0.05, **P < 0.01