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Fig. 5 | Cancer Cell International

Fig. 5

From: Long non‐coding RNA VPS9D1-AS1 facilitates cell proliferation, migration and stemness in hepatocellular carcinoma

Fig. 5

EGR1 transcriptionally activates SEC61A1 and VPS9D1-AS1. a qRT-PCR evaluated relative expression of VPS9D1-AS1 in HCC cells and normal liver epithelial cells. b Overexpression efficiency of EGR1 was verified in qRT-PCR. c Influence of EGR1 up-regulation on SEC61A1 and VPS9D1-AS1 expression was explored by qRT-PCR assay. d DNA motif of EGR1 and the binding sites of EGR1 on promoter of SEC61A1 and VPS9D1-AS1 were exhibited. e ChIP assay verified relative enrichment of SEC61A1 and VPS9D1-AS1 promoter pulled down by anti-EGR1. f Luciferase reporter assay revealed luciferase activity of wild and mutant SEC61A1 promoter and VPS9D1-AS1 promoter by treatment of pcDNA3.1-EGR1. *P < 0.05, **P < 0.01

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Cancer Cell International

ISSN: 1475-2867

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