Fig. 1
CircZDHHC20 was up-regulated and miR-144 was down-regulated in PE placental tissues. a qRT-PCR for circZDHHC20 expression in placental tissues from 26 PE patients and 15 healthy volunteers. b qRT-PCR for the levels of circZDHHC20 and linear ZDHHC20 mRNA after RNase R digestion. c The expression of circZDHHC20 and linear ZDHHC20 mRNA by qRT-PCR in reverse transcription using Random and Oligo(dT)18 primers. d CircZDHHC20 level by qRT-PCR in the nuclear and cytoplasm fractions of HTR-8/SVneo cells. 18S rRNA and U6 were used as internal controls. e The level of miR-144 in placental tissues from 26 PE patients and 15 healthy volunteers. f The correlation between circZDHHC20 level and miR-144 expression in placental tissues from 26 PE patients using the Spearman test. *P < 0.05