Fig. 6

CircZDHHC20 regulated GRHL2 expression through sponging miR-144. Correlations between GRHL2 mRNA expression and miR-144 level (a) or circZDHHC20 level (b) in 26 placental tissues from PE patients using the Spearman test. c, d GRHL2 protein level by western blot in HTR-8/SVneo cells transfected with negative control plasmid (vector), circZDHHC20 overexpression plasmid (circZDHHC20), circZDHHC20 + miR-con mimic, circZDHHC20 + miR-144 mimic, si-con, si-circZDHHC20, si-circZDHHC20 + anti-miR-con or si-circZDHHC20 + anti-miR-144. *P < 0.05