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Fig. 4 | Cancer Cell International

Fig. 4

From: SMAD6, positively regulated by the DNM3OS-miR-134-5p axis, confers promoting effects to cell proliferation, migration and EMT process in retinoblastoma

Fig. 4

MiR-134-5p is sequestered by DNM3OS in RB cells. a RT-qPCR measured DNM3OS expression levels in RB and normal tissue samples. b Pearson correlation test analyzed the correlation of DNM3OS and miR-134-5p expression. c Pearson correlation test analyzed the correlation of DNM3OS and SMAD6 expression. d RT-qPCR measured DNM3OS expression in four RB cell lines and a normal ARPE-19 cell line. e Nuclear/cytoplasmic fractionation localized DNM3OS in RB cells. f Luciferase reporter assay evaluated the relative luciferase activity of DNM3OS-WT and DNM3OS-Mut. g RT-qPCR detected the knockdown efficiency of DNM3OS. h RT-qPCR evaluated miR-134-5p expression after DNM3OS knockdown. Experiments were conducted thrice. *p < 0.05, **p < 0.01

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Cancer Cell International

ISSN: 1475-2867

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