Fig. 3

MiR-4319 was targeted by circATXN7, and repressed cell proliferation and invasion while boosted cell apoptosis in GC. a The cellular localization of circATXN7 in GC cells was determined by nuclear-cytoplasmic fractionation and FISH assays. b RNA pull-dwon assay was applied to screen out the potential miRNAs for circATXN7. c, d The expression of miR-4319 in GC tissues and cell lines was detected through RT-qPCR. e The efficiency of miR-4319 mimics was tested via RT-qPCR. f, g The interaction between circATXN7 and miR-4319 was verified by luciferase reporter assay and RNA pull-down assay. h The relationship between circATXN7 and miR-4319 levels in GC tissues was revealed via Spearman’s correlation analysis. i The effect of miR-4319 upregulation on cell proliferation was estimated via colony formation assay. j TUNEL assay was employed to measure the effect of miR-4319 mimics on cell apoptosis. k Transwell assay was applied to test the effect of miR-4319 overexpression on cell invasion. **P < 0.01