Fig. 5

P22077 enhances the cellular toxicity of Dox in HCC cells. a, b, SMMC-7721 and HepG2 cells were incubated with increasing concentrations of Dox (0, 0.01, 0.05, 0.1, and 0.5 µM) plus DMSO or 10 µM of P22077 for 48 h. The cell number was measured by a CCK-8 assay. c SMMC-7721 and HepG2 cells were treated with DMSO, P22077 (10 µM), Dox (0.5 µM) and their combination for 48 h. The cells were photographed. Dox is red fluorescent. d Immunofluorescence analysis of distribution of Dox (0.5 µM) in SMCC-7721 cell treated with or withoutP22077(10 µM) for 48 h. DAPI was labeled the nuclear DNA. All * P < 0.05 was considered significant