Fig. 6

P22077 regulated multiple essential biological processes in Huh7 cell. Huh7 cells were treated with P22077 for 24 h. The treated and non-treated cells were harvested. Total protein was extracted and subjected for mass spectrometry analysis. a The identified down-regulated protein was further analyzed by pathway enrichment to identified significantly affected pathways. b The identified up-regulated protein after P22077 treatment was analyzed for pathway enrichment. c The expression of H3 and H3K4me² was examined by Western Blot. d Cells were treated with P22077 and samples were resolved on SDS-PAGE gels and then transferred to nitrocellulose membranes for immunoblotting assays to detected the expression of H3, H3K4me², BAX was probed to detect apoptosis. GAPDH was a loading control