Fig. 1
lj-1-59 inhibits the proliferation of human melanoma cells. a Structure of lj-1-59. b SK-Mel-28 (upper panel) and SK-Mel-5 (lower panel) were prepared in 96-well plates. The cells were treated with increasing dose lj-1-59 for 0-72 h. Cell viability was determined by CCK-8 assay. The results represent the means (n = 6) ± SD. Significant differences were evaluated using Student’s t-test, and an asterisk (*) indicates a significant difference (p < 0.05). c The IC50 values of lj-1-59 in SK-Mel-28 (left panel) and SK-Mel-5 (right panel) cells were automatically calculated for 48 h by GraphPad Prism software. d SK-Mel-28 (left panel) and SK-Mel-5 (right panel) cells were prepared in 6-well plates. The cells were treated with increasing dose lj-1-59 for 24 h. After 2 weeks, the number of colonies was assessed and quantified as described in “Methods”. The data represent the mean (n = 4) ± SD, and an asterisk (*) indicates a significant difference (p < 0.05, Student’s t-test)