Fig. 4

Up-regulation of miR-223-3p reverses the effect of hsa_circ_0003159 on proliferation, migration, invasion and apoptosis in GC cells. a The expression of miR-223-3p was measured in NUGC-3 and AGS cells transfected with miR-NC, miR-223-3p, anti-NC or anti-miR-223-3p by qRT-PCR. b–g Cell proliferation (b, c), apoptosis (d, e), migration and invasion (f–h) and protein levels of CyclinD1, Cleaved-caspase-3 and MMP-9 (i) were detected in NUGC-3 and AGS cells transfected with vector, hsa_circ_0003159, hsa_circ_0003159 and miR-NC or miR-223-3p by CCK-8, flow cytometry, transwell and western blot assays, respectively. *P < 0.05