Fig. 2
Overexpression of Siah1 inhibits proliferation in CRC cells. a Ectopic expression of Siah1 in SW480 and HCT116 cells, analyzed by Western blot. α-Tubulin was used as a loading control. b, c Ectopic expression of Siah1 inhibits cell proliferation, as determined by MTT assays (b) and colony formation assays (c). d Overexpression of Siah1 inhibits SW480 and HCT116 cell growth in soft-agar assays. Only colonies containing > 50 cells were counted. Each error bar represents the mean ± SD from 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar: 50 μm. e SW480/Lv105 and SW480/Siah1 cells (2 × 106) were injected to the hindlimbs of nude mice (n = 6). The volumes of tumors were measured on the indicated days. Data points are displayed as the mean tumor volumes ± SD (lower panel). The upper panel shows tumors after inoculation. f The tumor histological sections were under H&E staining and IHC staining using an antibody against Siah1 and Ki-67 (left), right panel shows average percentage of staining cells among the total cell as the Ki-67 index. Scale bar: 50 μm