Fig. 4

miR-124-3p is a target of SNHG17 in BC cells. a The predicted binding sites of miR-124 on the sequence of SNHG17 (WT-SNHG17). The target sequences of the SNHG17 were mutated (MT-SNHG17). b Luciferase activity in MCF-7 and MDA-MB-231cells co-transfected with miR-124-3p mimics or miR-NC, and luciferase reporter vector containing WT-SNHG17 or MT-SNHG17. WT wild-type, MT mutant-type. c The expression of SNHG17 in nuclear and cytoplasmic of MCF-7 and MDA-MB-231 cells by qRT-PCR. d The interaction between miR-124-3p and SNHG17 in MCF-7 and MDA-MB-231 cells were tested by RIP experiment. e The expression of miR-124-3p in MCF-7 and MDA-MB-231 cells transfected with sh-NC or sh-SNHG17. f The expression of SNHG17 in MCF-7 and MDA-MB-231 cells transfected with miR-NC or miR-124-3p mimics. g Spearman’s correlation coefficient analysis between miR-124-3p expression and SNHG17 expression in 58 patients with BC. *P < 0.05, **P < 0.01