Fig. 2
From: Skp2 modulates proliferation, senescence and tumorigenesis of glioma
Downregulation of Skp2 attenuated in vitro and in vivo cell proliferation. a On Western blot analyses, the protein level of Skp2 in glioma cell lines (A172, U87, U118, U373, LNZ308, U138 and U343), as compared to normal astrocytes (AST), was enhanced. b Skp2 was successfully knocked-down by two shRNA fragments in U87, U138, and LNZ308 cells compared with the negative control shLuc. The protein levels of the downstream molecules p21Cip1/Waf1 and p27Kip1 were increased upon the knockdown of Skp2 in all three cell lines. c Cell proliferation was retarded slightly in U87, U138 and LNZ308 cells after the knockdown of Skp2. d The colony formation ability of the three cell lines was reduced after Skp2 knockdown. e, f The tumor weight and volume were smaller than the control group upon Skp2 knockdown in xenograft mouse models. **p < 0.01, ***p < 0.001