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Fig. 3 | Cancer Cell International

Fig. 3

From: Sirtuin 1 inhibits lipopolysaccharide-induced inflammation in chronic myelogenous leukemia k562 cells through interacting with the Toll-like receptor 4-nuclear factor κ B-reactive oxygen species signaling axis

Fig. 3

Influence of SIRT1 overexpression on inflammatory responses in k562 cells. a k562 cells were transfected with SIRT6-expressing plasmid/empty plasmid, followed by exposure to 10 μg/mL of LPS for 4 h or not. qPCR showed the mRNA expression of SIRT1 in k562 cells with different transfection and incubation. b, c Influence of LPS treatment and SIRT1 overexpression on the mRNA and protein expressions of inflammatory regulators determined using qPCR and ELISA. In qPCR, gene expression in each group was first normalized to the GAPDH gene, and then normalized to the data of the control group. d WB was performed to assess the protein expressions of SIRT1, IL-1β, IL-6, and TNF-α. e k562 cells were transfected with siRNA-NC or siRNA-SIRT1, followed by exposure to 10 μg/mL of LPS for 4 h or not. Influence of LPS treatment and SIRT1 silencing on the mRNA expressions of inflammatory regulators performed via qPCR. f KU812 cells were transfected with SIRT6-expressing plasmid/empty plasmid, followed by exposure to 10 μg/mL of LPS for 4 h or not. Influence of LPS treatment and SIRT1 overexpression on the mRNA expressions of inflammatory regulators in KU812 cells performed via qPCR. Data are expressed as mean ± standard deviations. *P < 0.05, **P < 0.01 and #P < 0.05, ##P < 0.01 relative to the control + pCDNA/siNC group and LPS + pCDNA/siNC group, respectively

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Cancer Cell International

ISSN: 1475-2867

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