Fig. 2

Knockdown of lncRNA NEAT1 suppressed cell proliferation and facilitated cell apoptosis in DLBCL. a RT-qPCR for NEAT1 expression in NEAT1 knockdown cells (shNEAT1) or negative control cells (shNC). **P < 0.01, ns = not significant vs. shNC, T-test. b Cell viability was measured in NEAT1 knockdown cells (shNEAT1) or negative control cells (shNC) with MTT assay. *P < 0.05, **P < 0.01 vs. shNC, T-test. c Colony formation assays were performed in NEAT1 knockdown cells (shNEAT1) or negative control cells (shNC). A representative image of the colony formation assay in OCI-Ly1 and SUDHL-4 cells is shown (left), and the total number of colonies per plate was counted (right). **P < 0.01 vs. shNC, T-test. d Flow cytometry analysis of annexin-V/PI staining of apoptotic cells following NEAT1 knockdown in OCI-Ly1 and SUDHL-4 cell lines. A representative image of FACS staining in OCI-Ly1 and SUDHL-4 cells is shown on the left, and the statistical data are shown on the right. **P < 0.01 vs. shNC, T-test. e TUNEL staining for OCI-Ly1 and SUDHL-4 shNEAT1 cell lines and their shNC lines. Representative images of TUNEL staining (left) and the statistical data (right) are shown. *P < 0.05, **P < 0.01 vs. shNC, T-test. f The mRNA level of GLI1 was assessed in OCI-Ly1 and SUDHL-4 shNEAT1 cell lines and their shNC lines by RT-qPCR. *P < 0.05 vs. shNC, T-test. g Immunoblotting and quantification of GLI1, cyclin D1, CDK4 and p27 (and β-actin as loading control) in OCI-Ly1 and SUDHL-4 shNEAT1 cell lines and their shNC lines. *P < 0.05, **P < 0.01 vs. shNC, T-test