Fig. 2

Circ_0072083 knockdown decreases the DDP resistance of NSCLC cells. a, b The level of circ_0072083 was detected in H522 and A549 cells transfected with si-NC or si-circ_0072083 by qRT-PCR. c–m H522 and A549 cells were treated with si-NC, si-circ_0072083, DDP + si-NC or DDP + si-circ_0072083. c The colony formation ability was detected in NSCLC cells through colony formation assay. d, e The apoptosis rate of NSCLC cells was evaluated by flow cytometry. f, g Western blot assay was carried out to detect the apoptosis-related markers in NSCLC cells. h, i Cell cycle of NSCLC cells was analyzed by flow cytometry. j, k The motility of NSCLC cells was detected through conducting transwell migration and invasion assays. l, m Western blot assay was performed to detect the protein expression of E-cadherin, N-cadherin and Vimentin in NSCLC cells, and GAPDH served as the internal reference in this study. *P < 0.05