Fig. 5

CBLL1 binds to miR-545-3p in NSCLC cells. a The 3′ UTR of CBLL1 possessed the complementary bases with miR-545-3p using Targetscan software. b, c Luciferase activity was determined in H522 and A549 cells co-transfected with miR-NC or miR-545-3p and CBLL1 3′ UTR WT or CBLL1 3′ UTR MUT reporter plasmid. d The protein expression of CBLL1 was examined in NSCLC samples and matching non-tumor tissues by western blot assay. e The level of CBLL1 was measured in BEAS-2B, H522 and A549 cells by western blot assay. f, g H522 and A549 cells were transfected with miR-NC, miR-545-3p, anti-miR-NC or anti-miR-545-3p, and Western blot was performed to detect the level of CBLL1 in the above NSCLC cells. h, i The expression of CBLL1 was detected in NSCLC cells transfected with si-NC, si-circ_0072083, si-circ_0072083 + anti-miR-NC or si-circ_0072083 + anti-miR-545-3p by western blot assay. *P < 0.05