Fig. 3

PEG3 is targeted by miR-21 and restoring PEG3 represses migration, proliferation and invasion as well as advances apoptosis of glioma cells. A, The binding site of miR-21 and PEG3 predicted by microRNA.org bioinformatics software. B, The target relationship between miR-21 and PEG3 confirmed by dual luciferase reporter gene assay. C, The expression of miR-21 and PEG3 mRNA in cells detected by RT-qPCR. D, Detection of PEG3 protein expression in cells by western blot assay. E, Cell proliferation tested by EdU assay. F, Detection of apoptosis by flow cytometry. G, Cell invasion tested by Transwell assay. H, Cell migration detected by scratch test. I, The protein expression of Bcl-2, Bax, MMP-2 and MMP-9 measured by western blot assay. N = 3. *p < 0.05 vs. the inhibitors NC group. ^#p < 0.05 vs. the OE-NC group. The measurement data were represented by mean ± standard deviation. Comparison between two groups were assessed by t test