Fig. 7

Depleted miR-21 and restored PEG3 raise CD8⁺ T proliferation, cell cytotoxic activity, and IFN-γ level as well as decline U87 cell activity and TGF-β1 level; exosomes enhances the volume of tumor, Ki67 and PCNA expression as well as reduces the percentage of CD8⁺ T cells in glioma mice. a The proliferation of CD8⁺T cells tested by CFSE. b Detection of glioma cytotoxic activity by LDH. c Cell activity of U87 cells tested by CCK-8 assay. d TGF-β1 and IFN-γ levels tested by ELISA. e Tumor growth curve of mice in each group. f Immunohistochemical staining of Ki76 and PCNA in mouse orthotopic transplantation tumor (25 μm). g Percentage of T lymphocytes in mouse spleen to CD8⁺T cells detected by flow cytometry. *p < 0.05 vs. the inhibitors NC group. ^$p < 0.05 vs. the OE-NC group. ^&p < 0.05 vs. the PBS group. ^#p < 0.05 vs. the +CD8 group. The measurement data were represented by mean ± standard deviation. Comparison among multiple groups were conducted by one-way ANOVA followed by Tukey’s multiple comparisons test