Fig. 4

Overexpression of circ_0007534 relieved the miR-206-induced effects on CC cells by acting as a sponge of miR-206. a The qRT-PCR demonstrated that the miR-206-mediated high miR-206 expression was inhibited by circ_0007534 in SiHa and HeLa cells. (b-d) MTT and colony formation assay proved that circ_0007534 promoted cell viability (b, c) and proliferation (d) by upregulating the expression of miR-206. (e–g) Flow cytometry (e) and western blot (f, g) were performed to verify the regulation of circ_0007534/miR-206 axis in cell apoptosis. h Transwell assay validated that circ_0007534 facilitated cell invasive ability by targeting miR-206. NC negative control, OD optical density, Bcl-2 B-cell lymphoma-2, Bax Bcl-2-Associated X, C-Caspase3 Cleaved-Caspase3, GAPDH glyceraldehyde-phosphate dehydrogenase. All assays were performed for three times with technical n = 3. Statistical analysis was conducted by Student’s t-test and one-way analysis of variance followed by Tukey’s test. *P < 0.05, **P < 0.01, ***P < 0.001