Fig. 6

Up-regulation of GREM1 reverted the effects of si-circ_0007534 on CC cells. a-c GREM1 transfection was shown to relieve the expression downregulation of GREM1 mRNA in qRT-PCR (a) and protein (b, c) in western blot caused by si-circ_0007534. d-f Cell viability inhibition in MTT (d, e) and proliferation repression in colony formation assay (f) by si-circ_0007534 were mitigated after transfection of GREM1. g-i The promotion of si-circ_0007534 on cell apoptosis was returned by the introduction of GREM1 through the analysis of flow cytometry (g) and western blot (h, i). j GREM1 transfection abolished the inhibition of cell invasion caused by si-circ_0007534 after performing transwell assay. NC negative control, GREM1 Gremlin1, OD optical density, Bcl-2 B-cell lymphoma-2, Bax Bcl-2-Associated X, C-Caspase3 Cleaved-Caspase3, GAPDH glyceraldehyde-phosphate dehydrogenase. All assays were performed for three times with technical n = 3. Statistical analysis was conducted by Student’s t-test and one-way analysis of variance followed by Tukey’s test. *P < 0.05, **P < 0.01, ***P < 0.001