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Fig. 3 | Cancer Cell International

Fig. 3

From: CDCA4 suppresses epithelial–mesenchymal transtion (EMT) and metastasis in Non-small cell lung cancer through modulating autophagy

Fig. 3

CDCA4 induces autophagy in NSCLC cells. a The effect of CDCA4 on the formation of acidic vacuoles was shown. Yellow to orange dots in the cytoplasm represented acidic vacuoles. Representative images were shown. b Electron microscopy showed the different autophagic activation in A549 CDCA4 overexpression cells. c Immunocytochemistry assay detected an elevated signal of LC3B and reduced signal of P62 in A549 CDCA4 overexpression cell. In H1299 CDCA4 knocking down cell a reduced signal of LC3B and elevated signal of LC3B were detected. d Western blotting analysis of P62, LC3B, Beclin-1, ATG5 and ATG7 protein expression. β-actin was used as a loading control. Data are means ± S.D. **P < 0.01, ***P < 0.005

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Cancer Cell International

ISSN: 1475-2867

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