Fig. 4

Activation or inhibition of autophagy alters the EMT, migration and invasion of NSCLC cells. A549 and H1299 cells were treated with dimethyl sulfoxide (control) or rapamycin (100 nM) for 48 h. a Western blotting analysis of P62, LC3B, E-cad, N-cad and Snail protein expression. β-actin was used as a loading control. b Comparison of the migration and invasion of A549 and H1299 cells using transwell compartments. A549 and H1299 cells were treated with phosphate-buffered saline (control) or 3-methyladenine (5 mM) for 48 h. c Western blotting analysis of P62, LC3B, E-cad, N-cad and Snail protein expression. β-actin was used as a loading control. d Comparison of the migration and invasion of A549 and H1299 cells using transwell compartments. Data are means ± S.D. *P < 0.05, **P < 0.01, ***P < 0.005