Fig. 4

Hsa_circ_0010235 served as a sponge for miR-433-3p. a Venn diagram of the overlapping target miRNAs of hsa_circ_0010235 predicted by circBank, CircInteractome and starbase. b QRT-PCR assay for the relative expression of miR-433-3p and miR-197-3p in H1299 and A549 cells transfected with pCD-ciR or hsa_circ_0010235. c The binding sites between hsa_circ_0010235 and miR-433-3p. d DLRA for the luciferase activity of H1299 and A549 cells co-transfected with miR-NC or miR-433-3p and wt-hsa_circ_0010235 or mut-hsa_circ_0010235. e RIP assay for the target relationship between hsa_circ_0010235 and miR-433-3p in H1299 and A549 cells. f QRT-PCR assay for the relative expression of miR-433-3p in 16HBE, H1299, A549, H1581 and H23 cells. g QRT-PCR assay for the relative expression of miR-433-3p in H1299 and A549 cells transfected with si-NC, si-hsa_circ_0010235#1, pCD-ciR or hsa_circ_0010235. h QRT-PCR assay for the relative expression of miR-433-3p in NSCLC tissues (n = 52) and normal tissues (n = 52). i Spearman’s correlation analysis for the expression levels of hsa_circ_0010235 and miR-433-3p in 52 NSCLC tissues. *P < 0.05