Fig. 6

TIPRL was a target of miR-433-3p. a The binding sites between miR-433-3p and TIPRL. b DLRA for the luciferase activity of H1299 and A549 cells co-transfected with miR-NC or miR-433-3p and wt-TIPRL 3′UTR or mut-TIPRL 3′UTR. c RIP assay for the target relationship between miR-433-3p and TIPRL in H1299 and A549 cells. d, e QRT-PCR and Western blot assays for the mRNA d and protein e expression levels of TIPRL in 16HBE, H1299, A549, H1581 and H23 cells. f QRT-PCR assay for the relative mRNA expression of TIPRL in H1299 and A549 cells transfected with anti-miR-NC or anti-miR-433-3p. g, i QRT-PCR assay for the relative mRNA g and protein i expression levels of TIPRL in H1299 and A549 cells transfected with miR-NC or miR-433-3p. h, j QRT-PCR assay for the relative mRNA h and protein j expression levels of TIPRL in H1299 and A549 cells transfected with anti-miR-NC or anti-miR-433-3p. k QRT-PCR assay for the relative mRNA expression of TIPRL in NSCLC tissues (n = 52) and normal tissues (n = 52). l Western blot assay for the protein level of TIPRL in NSCLC tissues and normal tissues. m Spearman’s correlation analysis for the expression levels of miR-433-3p and TIPRL mRNA in 52 NSCLC tissues. *P < 0.05