Fig. 7

Exosomes derived from SW480 cells down-regulated IRF4 expression in Tregs through miRNAs. SW480 cells and Tregs were co-cultured and incubated with GW4869. QRT-PCR (a) and WB ( b) were performed to assess the gene and protein expression of IRF4 in the Tregs. Exosomes were isolated from SW480 cells. c QRT-PCR was performed to detect the expression of miR-320c, miR-27a-3p and miR-30a-5p in the exosomes of SW480 cells. Tregs were incubated with the exosomes of SW480 cells. QRT-PCR (d) and WB (e) were performed to explore the gene and protein expression of IRF4 in the Tregs. SW480 cells were transfected with miR-30a-5p inhibitor, miR-320c inhibitor, miR-27a-3p inhibitor or the corresponding NC. Then, exosomes were isolated from the modified SW480 cells and incubated with Tregs. f, h and j QRT-PCR was performed to detect the expression of miR-30a-5p, miR-320c, miR-27a-3p and IRF4 in the modified SW480 cells. g, i and k WB was performed to detect the expression of IRF4 in the modified SW480 cells. (**P < 0.01, versus DMSO; ^##P < 0.01, versus SW480; ^$$P < 0.01, versus Ctrl; ^&&P < 0.01, versus inhibitor NC)