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Fig. 8 | Cancer Cell International

Fig. 8

From: IRF4 overexpression promotes the transdifferentiation of tregs into macrophage‐like cells to inhibit the development of colon cancer

Fig. 8

The expression of IRF4 was suppressed by miR-320c, miR-30a-5p and miR-27a-3p. Tregs were transfected with miR-320c mimic, mimic NC, miR-320c inhibitor or inhibitor NC. a QRT-PCR was performed to detect the expression of miR-320c and IRF4 in the modified Tregs. b WB was performed to explore the expression of IRF4 in the modified Tregs. Tregs were transfected with miR-30a-5p mimic, mimic NC, miR-30a-5p inhibitor or inhibitor NC. c QRT-PCR was performed to detect the expression of miR-30a-5p and IRF4 in the modified Tregs. d WB was performed to explore the expression of IRF4 in the modified Tregs. Tregs were transfected with miR-27a-3p mimic, mimic NC, miR-27a-3p inhibitor or inhibitor NC. e QRT-PCR was performed to detect the expression of miR-27a-3p and IRF4 in the modified Tregs. f WB was performed to explore the expression of IRF4 in the modified Tregs. (**P < 0.01, versus mimic NC; ##P < 0.01, versus inhibitor NC)

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