Fig. 6

PIK3CA was regulated by circ_0000020/miR-142-5p axis in glioma. a The 3′ UTR of PIK3CA contained 3 putative binding sites for miR-142-5p. b WT-PIK3CA or Mut-PIK3CA reporters (Mut1, Mut2, Mut3 and Mut1&2&3), together with miR-142-5p or miR NC, were co-transfected into U87 and U251 cells, respectively, and dual-luciferase assay was used to verify the targeting relationship between PIK3CA and miR-142-5p in gliomas. (n = 3). c, d U87 cells were transfected with miR-142-5p inhibitors, si-circ_0000020, si-circ_0000020 + miR-142-5p inhibitors; U251 cells were transfected with miR-142-5p mimics, circ_0000020 overexpression plasmid, circ_0000020 overexpression plasmid + miR-142-5p inhibitors. Then the expression of PIK3CA was detected by qRT-PCR (c) and Western blot (d), respectively. (n = 3). e qRT-PCR was used to detect the expression of PIK3CA in 50 cases of gliomas and adjacent brain tissues. f, g Person’s correlation coefficient was used to analyze the correlations between PIK3CA expression and circ_0000020 or miR-142-5p expression in glioma tissues, respectively. WT-PIK3CA: WT-pmirGLO-PIK3CA 3′ UTR, Mut1-PIK3CA: Mut1-pmirGLO-PIK3CA 3′ UTR, Mut2-PIK3CA: Mut2-pmirGLO-PIK3CA 3′ UTR, Mut3-PIK3CA: Mut3-pmirGLO-PIK3CA 3′ UTR, Mut1 & 2 & 3-PIK3CA 3′UTR: Mut1 & 2 & 3-pmirGLO-PIK3CA, N: normal tissue; T: tumor tissue; GAPDH: glyceraldehyde-3-phosphate dehydrogenase, Error bars represented the mean ± SD of at least three independent experiments; *P < 0.05, **P < 0.01, ***P < 0.001 and NS: P > 0.05