Fig. 3

LncRNA MEG8 interacts with miR-15a/b-5p in vitro. a Potential interaction between lncRNA MEG8 and miR-15a/b-5p was identified by the bioinformatic analysis using ENCORI (http://starbase.sysu.edu.cn/index.php). b The expression levels of miR-15a-5p and miR-15b-5p were tested by qPCR in the A549 cells treated with control mimic (miR-NC), or miR-15a/b-5p mimics. c Luciferase activities of MEG8 (MEG8 WT) and MEG8 with the miRNA-binding site mutant (MEG8 MUT) were determined by luciferase reporter gene assays in the A549 cells treated with control mimic (miR-NC), miR-15a-5p mimic, or miR-15b-5p mimic, respectively. d The interaction of MEG8 with wild type miR-15a-5p (Bio-miR-15a-5p), miR-15a-5p with the MEG8-binding site mutant (Bio-miR-15a-5p MUT), wild type miR-15b-5p (Bio-miR-15b-5p), and miR-15b-5p with the MEG8-binding site mutant (Bio-miR-15b-5p MUT) was examined by RNA-pull down followed by qPCR in the A549 cells. e The expression levels of miR-15a-5p and miR-15b-5p were measured by qPCR in the A549 cells infected with the lentiviral plasmids carrying MEG8 shRNA (shMEG8) or corresponding control shRNA (shNC). Data are presented as mean ± SD. Statistic significant differences were indicated: *P < 0.05, **P < 0.01