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Fig. 3 | Cancer Cell International

Fig. 3

From: Regulator of cullins-1 (ROC1) negatively regulates the Gli2 regulator SUFU to activate the hedgehog pathway in bladder cancer

Fig. 3

ROC1 modulation of SUFU protein levels. a, b Western blot. Expression of SUFU protein in 5637 (a) and T24 (b) cells after transfection with siROC1 or plasmid-ROC1 for 48, 72, 96, and 120 h. c qPCR. Bladder cancer 5637 cells were cotransfected with siRNA targeting ROC1 and SUFU and then subjected to qRT-PCR analysis of Gli1 and PTCH1 mRNA. d Western blot. Bladder cancer 5637 cells were cotransfected with siRNA targeting ROC1 and SUFU and then subjected to western blot analysis of Gli2 and SUFU protein. e, f Immunoprecipitation. Immunoprecipitation of SUFU from 5637 cells (e) and T24 cells (f) that were transfected with either siROC1 or pROC1. Nonspecific rabbit immunoglobulin G (IgG) was used as a negative control. Cell lysates were subjected to western blot analysis. Bars, SEM; *P < 0.05, ***P < 0.001

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Cancer Cell International

ISSN: 1475-2867

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