Fig. 1

miR-483 inhibits the EMT in osteosarcoma cells. a The relative expression of miR-483 in the osteosarcoma cell lines Saos-2, U2OS, and MG-63 was measured using RT-qPCR. The human normal osteoblast cell line hFOB.19 was the control cell line. The expression of miR-483 was normalized to U6. b The relative expression of miR-483 in normal tissues and osteosarcoma tissues excised from patients was assessed using RT-qPCR (n = 20). The expression of miR-483 was normalized to U6. c–h U2OS cells were un-transfected or transfected with a negative control miRNA (NC) or miR-483 mimics. c The relative expression of miR-483 in U2OS cells was measured using qRT-PCR and normalized to U6 expression. d–e The migration of U2OS cells was measured using a scratch wound healing assay (d) or transwell migration assay (e). Scale bar: 200 μm. f The invasion of U2OS was assessed using the transwell invasion assay. Scale bar: 200 μm. g The relative expressions of N-cadherin, E-cadherin, Vimentin, and Snail in U2OS cells were determined using qRT-PCR. The mRNA levels were normalized to the GAPDH mRNA. h The protein levels of N-cadherin, E-cadherin, Vimentin, and Snail in U2OS cells were examined using western blotting. GAPDH served as the input control. All experiments were performed for least three times in this study, which referred to biological replicates. Each biological replicate was conducted in 3 wells with cells of the same passage, which referred to technical replicates. Error bars denoted mean ± SD. p values were calculated using paired Student’s t-test (B) or one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test (A, C-H). ***p < 0.001, **p < 0.01 and *p < 0.05