Fig. 5

The lncRNA NEAT1 promotes the EMT in osteosarcoma cells by inhibiting miR-483.U2OS cells were transfected with a negative control plasmid (OE-NC), NEAT1 overexpression plasmid (OE-NEAT1) or NEAT1 overexpression plasmid plus miR-483 mimics. a, b The migration of transfected U2OS cells was measured using a scratch wound healing experiment (a) or transwell migration experiment (b). Scale bar: 200 μm. c The invasion of transfected U2OS cells was determined using transwell invasion assays. Scale bar: 200 μm. d The relative expression levels of N-cadherin, E-cadherin, Vimentin, and Snail in transfected U2OS cells were determined using qRT-PCR and normalized to the GAPDH mRNA. e The levels of N-cadherin, E-cadherin, Vimentin, Snail, STAT1, STAT3 and the phosphorylation of STAT3 in transfected U2OS cells were examined using western blotting. GAPDH was employed as the input control. All experiments described in this study were performed at least three times, which referred to biological replicates. Each biological replicate consisted of 3 wells with cells of the same passage, which referred to technical replicates. Error bars denoted mean ± SD. p values were calculated using one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test. ***p < 0.001, **p < 0.01 and *p < 0.05