Fig. 2
The CCA cell line exhibits decreased expression of miR-1182 and let-7a but increased NUAK1 expression. a Representative images of immunohistochemistry of NUAK1 expression in CCA tissue and normal tissues (×200). b The expression of miR-1182, let-7a and NUAK1 in CCA tissues (n = 56) and normal tissues (n = 32) determined by RT-qPCR. c The expression of miR-1182, let-7a and NUAK1 in different TNM stage (NI–II = 22; NIII–IV = 34) determined by RT-qPCR. d Pearson analysis of the correlation of the expression of miR-1182, let-7a and NUAK1 in CCA tissues (n = 56). e The expression of miR-1182, let-7a and NUAK1 in different cell lines determined by RT-qPCR. f The expression of NUAK1 in different cell lines determined by Western blot analysis; * p < 0.05 vs. normal cells or HIBEC cells. Statistical data were measurement data and expressed as mean ± standard deviation. Comparisons between two groups were analyzed by independent t-test. Comparisons among multiple groups were analyzed using ANOVA and followed by Tukey’s post hoc test. Pearson correlation was used to analyze the correlation between two groups. The experiment was repeated 3 times independently. ANOVA analysis of variance