Fig. 3

NUAK1 is predicted to be regulated by miR-1182 and let-7a. a Prediction of binding sites of miR-1182 and let-7a to NUAK1 mRNA by bioinformatics analysis. b Dual-luciferase reporter gene assay of relative luciferase activity of pNUAK1-WT and pNUAK1-MUT upon treatment with let-7a mimic or miR-1182 mimic. c RIP experiment of relative enrichment of NUAK1, miR-1182, and let-7a upon addition of IgG or Ago2 in HCCC-9810 and Huh28 cells. d RT-qPCR analysis of NUAK1 mRNA expression upon treatment with NC, let-7a mimic or miR-1182 mimic. e Western blot analysis of NUAK1 protein expression upon treatment with NC, let-7a mimic or miR-1182 mimic and corresponding quantification. ^* p < 0.05 vs. the NC group or IgG group. Statistical data were measurement data, described as mean ± standard deviation. Comparisons between two groups were analyzed by independent t-test. The experiment was repeated 3 times independently. NUAK1 novel (nua) kinase family 1, miR-1182 microRNA-1182, let-7a microRNA-let-7a, NC negative control