Table 1 Primer list
Gene | Forward primer | Reverse primer | Tm (°C) |
|---|---|---|---|
ABCA1 | TTCCCGCATTATCTGGAAAGC | CAAGGTCCATTTCTTGGCTGT | 57.9 |
ABCG8 | AGCCTCCTTGCTAGATGTGAT | GTCTCTCGCACAGTCAAGTTG | 57.9 |
ABCG5 | ACTGCTTCTCCTACGTCCTG | CTGTAGTTGCCAATCAGTCGG | 59.4 |
SCD1 | TCTAGCTCCTATACCACCACCA | TCGTCTCCAACTTATCTCCTCC | 60.3 |
FASN | AAGGACCTGTCTAGGTTTGATGC | TGGCTTCATAGGTGACTTCCA | 60.6 |
GSK3β | CCGACTAACACCACTGGAAGCT | AGGATGGTAGCCAGAGGTGGAT | 59.8 |
PFKFB3 | ATTGCGGTTTTCGATGCCAC | GCCACAACTGTAGGGTCGT | 58.8 |
HIF1A-F | ATCCATGTGACCATGAGGAAATG | TCGGCTAGTTAGGGTACACTTC | 60.3 |
GAPDH | GGAGCGAGATCCCTCCAAAAT | GGCTGTTGTCATACTTCTCATGG | 59.8 |